Part:BBa_K1696006

P170-RFP

P170 is a acid-induced promoter, however, its trans-acting protein, RcfB, is involved in the basal activity leading to the leakage[1]. To test whether the inducible promoter is leaky or not and characterize the lactate-sensing system's performance in E.coli, we add RFP reporter gene after P170 promoter. Red fluorescent protein (rfp), isolated from a sea anemone relative (Discosoma sp.), is an ideal reporter protein for living color. Because its fluorescence can be easily detected without any pretreatment[2].

Reference:

[1] Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.

[2] Wan H, He J, Ju B, et al. Generation of Two-color Transgenic Zebrafish Using the Green and Red Fluorescent Protein Reporter Genes gfp and rfp[J]. Marine Biotechnology, 2002, 4(2):146-154.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 999
  Illegal AgeI site found at 1111
  
• 1000
  COMPATIBLE WITH RFC[1000]